Fig. 2
From: NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin
Knockdown of NLK in NSCLC cell lines inhibits cell proliferation. a NLK protein levels were assessed by western blot analysis in 11 human NSCLC cell lines. H522, H2405, H1869, and H1581 were negative for NLK expression, whereas H2342, A549, SPC-A-1, SW900, SK-MES-1, H1299, and H661 cells all showed positive expression of NLK at various levels. α-tubulin was used as loading control. b Verification of NLK knockdown in A549 and SK-MES-1 cell lines by western blot using lentivirus-mediated shRNA. c, d MTS assay showing reduced cell growth in A549 and SK-MES-1 cells infected with NLK-shRNA. **p < 0.01. e, f Cell counting assay showing decreased cell numbers in A549 and SK-MES-1 cells infected with NLK-shRNA. **p < 0.01