Fig. 3
From: NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin
Effects of NLK silencing on cell cycle of NSCLC cell lines. a–d Flow cytometric analysis and quantitation of cell cycle in A549 (a, b) and SK-MES-1 cells (c, d) infected with control or NLK-shRNA. e Western blots showing that knockdown of NLK in A549 cells affects the expression of multiple cell cycle-related proteins including Cyclins, CDKs, and CDKIs. α-tubulin was used as loading control. f It is predicted that there might exist some correlation between JUN proteins and NLK. g Western blots showing that knockdown of NLK in A549 cells affects the expression and phosphorylation of Jun family members including c-Jun, JunB, and JunD. α-tubulin was used as loading control