Fig. 8
From: NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin
a NLK knockdown and metformin treatment reduce A549 cell stemness. Tumorsphere formation assay was performed in A549 infected with either scramble or NLK-shRNA. b Bar graph showing quantification results of numbers and diameter of spheres formed per well of each group. c Flow cytometric analysis of cell surface marker CD133 expression in A549 cells infected with scramble or NLK-shRNA. Bar graph showing quantification results of percentage of CD133+ cells. d Tumorsphere formation assay was performed in A549 treated with PBS or metformin. e Bar graph showing quantification results of numbers and diameter of spheres formed per well and quantification results of CD133+ cells percentage. f Both NLK knockdown and metformin treatment significantly decreased the expressions of Nanog, c-Myc and KLF4 in A549 cells