Fig. 2

Decreased in vitro blinatumomab-mediated lysis of ALL blasts by patient CD3-positive T cells. a PD-1 expression on peripheral CD3-positive T cells was compared to PD-1 expression on healthy donor CD3-positive T cells showing no detectable difference. b The patient’s ALL blasts were cocultured either with healthy donor CD3-positive T cells (upper panels) or the patient’s own CD3-positive T cells (lower panels) with either control-Bite® (left panels) or blinatumomab (right panels) and analyzed by flow cytometry after 3 days. c Specific lysis was calculated as one minus the ratio of CD19-positive cells treated with blinatumomab and CD19-positive cells treated with control-Bite®. Healthy donor CD3-positive T cells showed efficient lysis of our patient’s ALL blasts (specific lysis 93.6 %) whereas CD3-positive T cells from our patient showed inefficient lysis of autologous ALL blasts (specific lysis 8.5 %). d IFN-γ concentration in cell culture supernatants were considerably lower for our patient’s T cells cocultured with his ALL blasts, whereas coculture of healthy donor T cells and ALL blasts from our patient led to considerable IFN-γ production. Cell cultures with control-Bite® did not show any IFN-γ production