Fig. 3
Sialidase expression and activity. a Total RNA isolated from platelets was reverse-transcribed, and the resulting cDNA was used as a template for amplification with primers corresponding to NEU1, NEU2, NEU3, and NEU4, as well as HPRT as a housekeeping gene control. The mRNA level for each sialidase was normalized to HPRT. b Flow cytometry analysis of NEU1 and NEU3 expression on platelet surfaces. The data are presented as MFI. c NEU1 localization in the platelets from the patients and the control group. The fluorescence intensity of NEU1 in the PT platelets was slightly elevated after permeabilization, and its distribution was correlated with that of β-Gal. The NEU1 signals of the platelets from the control group were weaker than those from the PT patients, especially when not permeabilized. d Correlation between the increase in RCA-I and NEU1 expression in the PT patients. R 2 = 0.408. Correlation between NEU1 and RVA-I MFI was determined by Spearman’s correlation coefficient; P = .585, P = .17. e Sialidase activity inside and on the surfaces of platelets from the PT patients. The total sialidase activity in platelets was measured after detergent permeabilization. f Total NEU1 expression of the platelets from the PT patients and the control group