Fig. 6
From: Small PARP inhibitor PJ-34 induces cell cycle arrest and apoptosis of adult T-cell leukemia cells
PJ-34 causes DNA damage and p53 pathway activation even in MT-2 and C91PL cells. a Western blot of p-H2AX, pS15-p53 and p21 in MT-4 cells treated with DMSO or PJ-34. Actin was used to confirm equal loading. b Western blot of p53 in MT-2, MT-4 and C91PL cells after treatment with DMSO or PJ-34. c–e Cells were treated with DMSO or PJ-34 and the changes of p53 target genes Bax (c), MDM2 (d), and GADD45a (e) were analyzed using real-time RT-PCR. Real-time PCR was performed in duplicate, and data were normalized to GAPDH expression. Results were presented as mean ± SD from two independent experiments