Fig. 7
From: Small PARP inhibitor PJ-34 induces cell cycle arrest and apoptosis of adult T-cell leukemia cells
The defect of caspase-3 activation in MT-2 and C91PL contributes to the resistance of PJ-34 in MT-2 and C91PL cells. a MT-4 and MT-2 were treated with DMSO or PJ-34. Cells were subsequently stained with JC-1, and mitochondrial membrane potential (ΔΨm) was measured by FACS analysis. Representative results of two experiments are shown here. b Western blot of analysis of Bcl-2, Bax, and Bcl-xL in MT-2, C91PL, and MT-4 after treatment with DMSO or PJ-34. c MT-2, MT-4, and C91PL were treated with DMSO or PJ-34 for 3 days. Then, total cell extracts were probed for caspase-8, caspase-3, and PARP-1 expression. d Relative expression of FLIP and XIAP were compared using real-time RT-PCR among resistant cell lines MT-2, C91PL, and other sensitive cell lines. Results were presented as mean ± SD from two independent experiments