Fig. 3
From: Roles of palmitoylation and the KIKK membrane-targeting motif in leukemogenesis by oncogenic KRAS4A
Effects of palmitoylation on signaling transduction of oncogenic NRAS vs. KRAS4A. Serum-starved lysates of NIH3T3 cells expressing GFP, KRAS4AG12D, KRAS4AG12D/C180S, NRASG12D, or NRASG12D/C181S were analyzed by western blotting. a Phospho-c-RAF, MEK, and ERK1/2 antibodies were used to detect the effects of palmitoylation deficiency on the MEK-ERK signaling pathway. b Phospho-AKT and S6RP antibodies were used to detect the effects of palmitoylation deficiency on the PI3K-AKT signaling pathway. Expression levels of RAS, GFP, and β-Actin were detected as loading controls