Fig. 4

FISH validation of Igh locus translocations. a, b Inactivation of Xrcc4 and Trp53 via Cγ1-cre leads to reciprocal Igh-c-myc translocation in G1XP lymphoma (46 J). a Upper left: Diagram of Igh FISH probes. Bottom left: An example of metaphase Igh FISH image. An intact Igh shows co-localized red and green signals while a translocated locus appears as split red and green signals on different chromosomes (white arrow). Upper right: Diagram of c-myc FISH probes. Bottom right: An example of metaphase FISH showing c-myc locus translocation (yellow arrow) and an intact c-myc locus. b Left: A metaphase FISH image showing the t(12;15) translocation with 3′Igh (red) and 3′c-myc (green) probes juxtaposed on the derivative chr12 (white arrow). Right: A metaphase FISH image showing the t(15;12) translocation with 5′c-myc (red) and 5′Igh (green) probes juxtaposed on the derivative chr15 (yellow arrow). A normal chr12 and chr15 are also present in the metaphase. c Left: a metaphase FISH image showing the Igh locus translocations in G1XP lymphoma (119 J) with 3′ and 5′Igh probes split. Right: a metaphase FISH image showing the intact c-myc locus with 3′ and 5′ probes co-localized in the same lymphoma sample