Fig. 3
From: Cancer-associated fibroblasts promote the progression of endometrial cancer via the SDF-1/CXCR4 axis
CAFs promoted EC progression via the SDF-1α/CXCR4 axis. a Cytokines secreted by EC cells, NFs, and CAFs were measured by ELISA. b SDF-1α protein concentration in the conditioned media was measured for 96 h by ELISA. c The effects of SDF-1α-induced proliferation of HEC-1B cells were inhibited by AMD3100. Three independent experiments were performed in triplicate. Data are expressed as means ± SDs. *P < 0.05; **P < 0.01; ***P < 0.001. d MMP-2 and MMP-9 gelatinase activity in the conditioned media was analyzed by zymography. e, f The effects of SDF-1α-induced migration and invasion of EC cells were inhibited by AMD3100 (magnification ×200). g Western blot analysis detection of the phosphorylation of Ark and Erk protein expressions in HEC-1B cells