Fig. 4

Analysis of trilineage differentiation of c-kit⁺ HSPCs on β-TCP scaffolds in vitro. a Differentiation potential of c-kit⁺ cells cultured for 4 and 14 days on β-TCP scaffolds with and without collagen I/III gel/Matrigel® in the presence of MSC support: granulocytes (Gr1⁺CD11b⁺), monocytes (Gr1⁻CD11b), erythroid precursor cells (Gr1⁻CD11b⁻CD3⁻CD19⁻), and T cells (Gr1⁻CD11b⁻CD3⁺). Percentage of viable cells is shown as mean ± SD of three independent experiments. b SEM of c-kit⁺ cells cultured on 500-μm β-TCP/Matrigel® scaffolds. Very left column highlights cell spreading and detailed cell morphology of day 4 and day 14 cultures. C-kit⁺-derived cells (red arrows) are in close contact to MSCs (white arrows). Cell extensions at day 4 in detail (green arrow). View from the interior of the β-TCP macropores at day 14 (asterisk)