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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites

Fig. 5

Histomorphology. 500μm or 800 μm β-TCP scaffolds were pre-seeded with mBMSC in the presence or absence of Matrigel®. Scaffolds were transplanted subcutaneously into C57B1/6 mice and analyzed for hematopoietic cell recruitment to sites of extramedullar hematopoiesis and hematopoietic tissue formation after 4 and 8 weeks. a Hematoxilin-eosin (HE) staining of the explanted scaffolds 8 weeks after transplantations. Magnification 100x. b Higher magnification of the explanted scaffolds after 8 weeks. Magnification 200x. c Immunohistochemistry for the endothelial surface marker CD31 and the pan-hematopoietic marker CD45 in explanted scaffolds 8 weeks after transplantation. d HE staining and by SEM for the identification of osteoclasts at the border of the β-TCP scaffold macropores in explants 4 weeks after transplantation

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