Fig. 6From: An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites a SEM imaging of the explanted scaffolds 4 weeks after transplantation. White arrows: erythrocytes; black arrows: T cells; red arrows: thrombocytes; gray arrow: neutrophils/granulocytes; asterisk: β-TCP. b-c Gating strategy for the flow cytometry: Hematopoietic stem (LSK) and progenitor cells (LK) were identified by being negative for lineage markers and by the expression of Sca1 and c-kit. Lineages were identified by the following stainings: granulocytes (Gr1+CD11b+), monocytes (Gr1−CD11b−), erythroid precursor cells (Gr1−CD11b−CD3−CD19−), T cells (Gr1−CD11b−CD3+), B cells (Gr1−CD11b−CD19+). d Flow cytometry analysis of the explanted scaffolds 4 weeks after transplantations. Surface marker expression is shown as a percentage of the viable cell population; mean ± SD of three independent experimentsBack to article page