Fig. 5

a Schematic representation of the AML post MDS mouse model. The characteristics of these models have previously been published ^[24-26;29]; b Decrease of the survival of AML post MDS mice compared to the survival of the funders, _MRP8NRASD12 and _MRP8hBCL-2; c Increased level of Lin-Sca1+-cKit+ (LSK) cells in the BM; d Decreased level of apoptosis in the liver by whole body SPECT using ⁹⁹Tc-Annexin (liver and spleen region located above the kidneys); e Validation of microarray data: Decreased levels of MEK isoforms in the AML post MDS spleen cells compared to FVB/N control mice by the nanofluidic proteomic analysis; f The disease can be transplanted in normal FVB/N irradiated syngeneic mice with either the Sca1+ cells of the spleen or BM of the AML post MDS mice resulting in 54 % blast infiltration in the BM blasts as in AML post MDS mice.^[24-26;29]