Fig. 1
From: Haplo-insufficiency of both BubR1 and SGO1 accelerates cellular senescence
BubR1 +/− SGO1 +/− MEFs exhibit senescent-like morphologies at an accelerated rate. Primary MEFs of various genetic backgrounds (wild-type, BubR1 +/−, SGO1 +/−, and BubR1 +/− SGO1 +/−) were cultured in DMEM supplemented with FBS and antibiotics as described in the Methods section. Serial cultures were carried out at a density of 106 cells/100-mm dish every 7 days. At each passage, images of MEFs were recorded with a light microscope. Images of MEFs of wild-type (WT), BubR1 +/−, SGO1 +/−, and BubR1 +/− SGO1 +/− at passages 4, 6, and 8 are shown