Fig. 3

Intracellular BTK expression and B-cell functional responses. A blood sample collected from the patient on day 279 was used for these assays. a Flow cytometric analysis of BTK expression in monocytes and B-cells. Gray histograms indicate isotype control and black histograms indicate BTK expression. b CFSE-labeled proliferation induced by CD40L and CpG or CD40L and IL-21 in CD19⁺ B-cells. Numbers in the plots indicate the percentage of divided cells. c Immunoglobulin production induced by CD40L and IL-21 or CpG