Fig. 3

a The combination of rapamycin and FAK down-regulation induced enhanced G₀/G₁ cell cycle arrest in REH cells. REH-empty vector or REH-FAK shRNA cells were cultured with or without rapamycin (100 nM). After 48 h, the cell cycle was analyzed in these cells. The statistical significance of differences between populations in the G₀/G₁ and S phases of the cell cycle induced by either rapamycin or FAK down-regulation alone or by combined treatment with both was determined through one-way analysis of variance (ANOVA) followed by Bonferroni’s multiple comparison tests. The results represent the mean ± S.D. of three experiments performed in triplicate. *p < 0.05; Rapa, rapamycin. b The induction of apoptosis in REH cells by rapamycin and FAK down-regulation. REH-empty vector or REH-FAK shRNA cells were cultured with or without rapamycin (100 nM). After 30 h, cell apoptosis was analyzed using the annexin V/PI apoptosis detection kit. The combination of rapamycin and FAK down-regulation induced more cell apoptosis in REH cells than in rapamycin treatment alone