Fig. 2
Anti-proliferative effects of GMR CAR T cells on MO7e, K562, and Daudi cells. a Flow cytometric analysis of MO7e cells co-cultured with mock T cells or GMR CAR T cells at an E:T ratio of 2:1. After 4 days, cultured cells were stained with either PE-conjugated anti-CD33 or PE-conjugated anti-CD19 and APC-conjugated CD3 mAbs. b % growth (proportion of initial cell numbers) of MO7e cells co-cultured with mock T cells or GMR CAR T cells from three healthy donors. The culture of 1 × 105 MO7e cells alone generated 3.6 × 105 cells in the presence of 10 ng/ml GM-CSF after 4 days. c % growth (proportion of initial cell numbers) of K562 cells co-cultured with mock T cells or GMR CAR T cells from three healthy donors. The culture of 1 × 105 K562 cells alone generated 8.0 × 105 cells after 4 days. d % growth (proportion of initial cell numbers) of Daudi cells co-cultured with mock T cells or GMR CAR T cells from three healthy donors. The culture of 1 × 105 Daudi cells alone generated 9.4 × 105 cells after 4 days