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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34+ cells of juvenile myelomonocytic leukemia

Fig. 3

Inhibitory effects of GMR CAR T cells on colony growth of JMML, normal CB, and normal BM CD34+ cells. Either GMR CAR T cells or mock T cells were incubated with JMML PB CD34+ cells (n = 5), normal CB CD34+ cells (n = 3), or normal BM CD34+ cells (n = 3) at E:T ratios of 1:1 and 1:4, in the presence of SCF + TPO + IL-3 for 2 days. Cells were then cultured on methylcellulose in media supplemented with GM-CSF, SCF, IL-3, and erythropoietin. After 14 days, the total number of colonies (GM colonies plus erythroid colonies) was calculated for each. Values are expressed as percentages of the total colony numbers obtained by culture in the absence of T cells. The total numbers of colonies grown by 500 JMML PB CD34+ cells, CB CD34+ cells, and BM CD34+ cells in the absence of T cells were 45.4 ± 11.6, 81.3 ± 19.1, and 43.6 ± 0.8, respectively

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