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Table 1 Effects of GMR CAR-T cells on expansion of JMML CD34+ cells under stimulation with SCF+TPO on AGM-S3 cells

From: Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34+ cells of juvenile myelomonocytic leukemia

  None Healthy T cells JMML T cellsa
  Mock 1:1 CAR 1:1 Mock 1:1 CAR 1:1
Patient no. 5      
 CD34+ cells (×104) 8.85 ± 0.44 7.75 ± 0.62 2.57 ± 0.33 6.73 ± 0.52 0.88 ± 0.04
 CD34+CD38 cells (×104) 3.78 ± 0.19 3.20 ± 0.26 0.04 ± 0 2.80 ± 0.22 0 ± 0
 CD34+CD38+ cells (×104) 5.07 ± 0.25 4.56 ± 0.37 2.53 ± 0.33 3.93 ± 0.30 0.88 ± 0.04
Patient no. 6      
 CD34+ cells (×104) 9.38 ± 0.77 8.15 ± 0.67 1.91 ± 0.17* 7.50 ± 0.49 2.50 ± 0.20*
 CD34+CD38 cells (×104) 5.45 ± 0.45 4.65 ± 0.38 0.30 ± 0.03* 4.52 ± 0.30 0.55 ± 0.04*
 CD34+CD38+ cells (×104) 3.93 ± 0.32 3.50 ± 0.29 1.62 ± 0.15* 2.98 ± 0.19 1.96 ± 0.16*
  1. Patient no. 5 monosomy 7, patient no. 6 trisomy 8 plus PTPN11 mutation, GMR GM-CSF receptor, CAR chimeric antigen receptor, JMML juvenile myelomonocytic leukemia, SCF stem cell factor, TPO thrombopoietin
  2. * p < 0.0001, significant different from mock T cells
  3. aT cells were obtained from patient no. 4 with PTPN11 and SETBP1 mutations after unrelated CBT