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Table 1 Effects of GMR CAR-T cells on expansion of JMML CD34+ cells under stimulation with SCF+TPO on AGM-S3 cells

From: Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34+ cells of juvenile myelomonocytic leukemia

 

None

Healthy T cells

JMML T cellsa

 

Mock 1:1

CAR 1:1

Mock 1:1

CAR 1:1

Patient no. 5

     

 CD34+ cells (×104)

8.85 ± 0.44

7.75 ± 0.62

2.57 ± 0.33

6.73 ± 0.52

0.88 ± 0.04

 CD34+CD38 cells (×104)

3.78 ± 0.19

3.20 ± 0.26

0.04 ± 0

2.80 ± 0.22

0 ± 0

 CD34+CD38+ cells (×104)

5.07 ± 0.25

4.56 ± 0.37

2.53 ± 0.33

3.93 ± 0.30

0.88 ± 0.04

Patient no. 6

     

 CD34+ cells (×104)

9.38 ± 0.77

8.15 ± 0.67

1.91 ± 0.17*

7.50 ± 0.49

2.50 ± 0.20*

 CD34+CD38 cells (×104)

5.45 ± 0.45

4.65 ± 0.38

0.30 ± 0.03*

4.52 ± 0.30

0.55 ± 0.04*

 CD34+CD38+ cells (×104)

3.93 ± 0.32

3.50 ± 0.29

1.62 ± 0.15*

2.98 ± 0.19

1.96 ± 0.16*

  1. Patient no. 5 monosomy 7, patient no. 6 trisomy 8 plus PTPN11 mutation, GMR GM-CSF receptor, CAR chimeric antigen receptor, JMML juvenile myelomonocytic leukemia, SCF stem cell factor, TPO thrombopoietin
  2. * p < 0.0001, significant different from mock T cells
  3. aT cells were obtained from patient no. 4 with PTPN11 and SETBP1 mutations after unrelated CBT