Fig. 4

Effect of ATO on downmodulation of HHEX expression elicited by PML-RARα induction in PR9 cells (top and middle panels) and on HHEX expression observed in NB4 cells (bottom panel). Top panels (a, b): PR9 cells have been grown either in the absence (c) or in the presence of ZnSO₄ for 48 h (left and right panels), in the presence of ATO for different times (from 6 to 48 h, right panel), or in the presence of ZnSO₄ + ATO for different time points (from 6 to 48 h, left panel) and HHEX, PML-RARα, or RARα expression were assessed by WB. A representative WB is shown at the top of each panel and the quantitative evaluation (mean ± SEM in three separate experiments). Bottom panel (c): NB4 cells have been grown either in the absence (c) or in the presence of ATO for different times (from 6 to 48 h) and HHEX expression was analyzed by WB