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Fig. 8 | Journal of Hematology & Oncology

Fig. 8

From: PML-RAR alpha induces the downmodulation of HHEX: a key event responsible for the induction of an angiogenetic response

Fig. 8

Effect of HHEX overexpression on the capacity of PR9 cells to express VEGF-A and to differentiate in response to VitD3. PR9 cells have been transfected with a control empty vector (pTWEEN) or with this vector containing the human HHEX gene (pHHEX) and then a analyzed for HHEX expression by Western blotting (top panel, left) and for VEGF-A RNA expression by realtime-PCR (top panel, right); b pTWEEN PR9 cells and pHHEX PR9 cells have been treated or not with Zn2+ for 24 h and then grown in the absence or in the presence of 1α25OH-VitD3 and evaluated at different times for the number of viable cells (middle panels) and at day 3 of culture for the expression of CD11 and CD14 membrane antigens (low panels). The results of the three experiments ± SEM are reported in the figure. For PR9 cells treated with Zn2++VitD3, the difference between pTWEEN PR9 and pHHEX PR9 cells for both CD11b and CD14 expression was highly significant (p ≤ 0.01)

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