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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Rheb1 promotes tumor progression through mTORC1 in MLL-AF9-initiated murine acute myeloid leukemia

Fig. 3

Rheb1 deletion impairs LSC function. a The differentiation profile of AML cells (left) and the percentages of subpopulations (right) using c-Kit and Gr-1 surface markers (n = 4). b The absolute number of these subpopulations in the BM (n = 4). c The absolute number of L-GMPs in the BM (n = 4). d, e The enrichment of selected gene targets for HOXA9 and MEIS1, NUP98-HOXA9 fusion genes (d), and HSC downregulation-related genes (e). The NES (normalized enrichment score) and P values are indicated in each plot. f The mRNA expression of the indicated genes assessed using RT-PCR. g, h Colony formation of GFP+ AML cells (g) and GFP+ K+G AML cells (h) that were sorted, replated in semisolid medium, and cultivated for 8 days prior to counting. The data show the mean colony numbers ± SEM. All experiments were performed at least three times. The data represent the mean numbers ± SEM. i The survival curve of Rheb1 fl/fl and Rheb1 Δ/Δ AML mice using Kaplan-Meier analysis (n = 6). Approximately 1000 P3 K+G cells from the two groups were transplanted into sub-lethally irradiated recipient mice (P4). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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