Fig. 4

Rheb1 deletion arrests cell cycle progression and increases the apoptosis of AML cells. a Upregulated (left) and downregulated (right) GO terms of differentially expressed genes in Rheb1 ^Δ/Δ GFP⁺ cells vs. the WT control. b Enrichment of the cell cycle process. NES (normalized enrichment score) and P values are indicated in each plot. c, d The cell cycle status of GFP⁺ (c) and K⁺G⁻ cells (d), n = 4. e, f GFP⁺ cells (e) and K⁺G⁻ cells (f) were sorted, and their RNAs were isolated for indicated gene analysis using RT-PCR. The data represent the mean numbers ± SEM. g, h The apoptosis status of GFP⁺ (g) and K⁺G⁻ cells (h), n = 4. i, j GFP⁺ cells (i) and K⁺G⁻ cells (j) were sorted, and RNAs were isolated and analyzed using RT-PCR for the indicated genes. The data represent the mean numbers ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001