Fig. 4

NFκB signaling pathway is strikingly activated in P-MSCs. a 3D profile of RT² Profiler™ PCR Array of Human Toll-Like Receptor Signaling Pathway. b Activation of NF-κB in P-MSC was determined by Western blotting. The fold difference in band intensities was quantified and indicated under the image using the software of AlphaView. c Representative pictures of cellular immunofluorescence staining for nuclear p65 in MSCs or P-MSC at 2 h. Immunocytochemistry staining was performed using an anti-p65 antibody (red) and Hoechst (blue) for nuclear staining. d Relative mRNA expression of IL-6, IL-8, and MCP-1. MSCs were pre-treatment with or without the NF-κB inhibitor (PDTC, 10 μM) at 37 °C for 1 h and then incubated with 200 μg/ml A549 cell-derived exosomes at 37 °C for 24 h (*P < 0.05, **P < 0.01, ***P < 0.001)