Skip to main content
Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway

Fig. 5

Exosomes trigger cytokine production in MSCs through TLR2. a The expression level of TLR 1 ~ 10 of MSCs. GAPDH was used as control. b mRNA expression changes of TLR2, 7, 8 were detected by RT-PCR after treatment with exosomes. c mRNA expression changes of IL-6, IL-8, and MCP-1 of MSCs. MSCs were pre-treated withTLR2 neutralizing antibody or mouse IgG2b isotype control at 37 °C for 1 h and then incubated with exosomes for 24 h. d Western blotting analysis of NF-κB activation in P-MSCs. MSCs treated with mouse IgG2b isotype control was used as control. e The interfering efficiency of three pairs of TLR2 siRNAs was measured by RT-PCR. f mRNA expression changes of IL-6, IL-8, and MCP-1. MSCs were stimulated with exosomes after knockdown of TLR2 by siRNA for 24 h (*P < 0.05, **P < 0.01, ***P < 0.001)

Back to article page