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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway

Fig. 6

Hsp70 on exosomes contribute to cytokine production through TLR2. a mRNA expression changes of IL-6, IL-8, and MCP-1. Exosomes were pre-treated with proteinase K (5 μg/ml) at 37 °C for 1 h and then treated at 95 °C for 10 min after proteinase K treatment to abolish its activity. Proteinase K treatment alone was used as another control. b mRNA expression changes of IL-6, IL-8, and MCP-1. Exosomes were pre-treated with or without anti-HSP70 antibody at 37 °C for 1 h. 10 μg/ml anti-HSP70 was used for blocking. c mRNA expression changes of IL-6,IL-8, and MCP-1 in MSCs treated with different concentrations of rHSP70 proteins for 24 h. d mRNA expression changes of IL-6, IL-8, and MCP-1 in MSCs treated with rhHSP70 after knockdown of TLR2 by siRNA for 24 h (*P < 0.05, **P < 0.01, ***P < 0.001)

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