Fig. 2

Differences in cell-cell interaction with different feeder layers. a The percentage of HPCs with elongated morphology was scored as described before [6]. Particularly, co-culture with MSCs stimulated cellular elongation (*P < 0.05, n = 3—each with three biological replicates for MSCs and iPS-MSCs). b Gene expression of relevant genes for cellular interaction with HPCs were analyzed in MSCs, iPSCs, and iPS-MSCs. Overall, all genes were expressed at very similar levels in primary MSCs and iPS-MSCs—except for Laminin β1 (LAMB1; higher expressed in iPS-MSCs; **P < 0.01) and vascular cell adhesion molecule 1 (VCAM1; higher expressed in MSCs; **P < 0.01). c Mean fluorescence intensity of VCAM1 (CD106) expression in flow cytometric analysis (three biological replicates for MSCs and iPS-MSCs). Mean ± S.D. is depicted