Fig. 5

Low HOTAIRM1 expression is attributed to the reduced PU.1 expression. a HOTAIRM1 and PU.1 mRNA expression levels in representative AML cell lines. b Low levels of HOTAIRM1 and PU.1 in APL patients, as compared with non-APL patients (GSE10358). c ChIP-qPCR showing the binding of PML-RARα on the HOTAIRM1 promoter, the PU.1 promoter (as a positive control), and the negative site. d The effect of PML-RARα on promoter activity of HOTAIRM1. The pGL3-HOTAIRM1 plasmid was co-transfected with 200 ng expression vectors (pcDNA3.1 alone, PML-RARα alone, PU.1 alone, PU.1 and PML-RARα together, respectively) into HEK-293T cells. e The Pearson correlation between PU.1 and HOTAIRM1 expression in an AML patient cohort (GSE10358). f The up-regulation of HOTAIRM1 after PU.1 overexpression. NB4 cells were harvested at 48 h post-transduction with the MigR1 empty vector or the MigR1-PU.1 vector. The expression level of PU.1 was detected by western blotting. The mRNA expression levels of HOTAIRM1 and ITGAM were detected by RT-qPCR. GAPDH was used for normalization. The data represent the mean of three replicates ± SD. *p < 0.05; **p < 0.01