Fig. 3

Impact of azacytidine on blood cell counts and immune recovery. Balb/cJ mice were injected i.v. with 10.10⁶ bone marrow cells and 70.10⁶ splenocytes from B10.D2 donor mice after lethal irradiation. Mice were then given (or not) azacytidine (AZA, 0.5 mg/kg or 2 mg/kg), administered subcutaneously every 48 h from day +10 to day +30. a–c Impact of AZA on total white blood cell counts (a), total lymphocyte counts (b) and hemoglobin levels (c). d–g FACS analyses performed on mice blood at various time points showing lower counts and proliferation of CD4⁺Tconvs (d, f) and of CD8⁺ T cells (e, g) during AZA administration but higher CD4⁺Tconvs (F) and of CD8⁺ T cells proliferation after AZA discontinuation. Results are expressed as median, 25th and 75th percentiles of the distribution (boxes), and whiskers extending to the 5th and 95th percentiles. *P < 0.05; **P < 0.01; ***P < 0.001