Fig. 1From: Effective adoptive immunotherapy of triple-negative breast cancer by folate receptor-alpha redirected CAR T cells is influenced by surface antigen expression levelConstruction and expression of folate receptor-alpha (FRα)-specific chimeric antigen receptor (CAR). a Schematic representation of MOv19-based FRα CAR constructs containing the CD27 co-stimulatory module in combination with the CD3ζ cytosolic domain. b Primary human CD3 T cells can efficiently express FRα-specific CAR. Expression was detected via PE-conjugated goat anti-mouse F(ab′)2 fragment and measured by flow cytometry. c Compared to untransduced (UNT) T cells, transduced T cells consisted of CD4- and CD8-positive cells with both subsets expressing FRα CAR. FRα CAR expression was detected via biotin-labeled recombinant FRα protein staining followed by streptavidin-PE after transduction with lentivirus. Transduction efficiencies are indicated with the percentage of CAR expression in parenthesesBack to article page