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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Effective adoptive immunotherapy of triple-negative breast cancer by folate receptor-alpha redirected CAR T cells is influenced by surface antigen expression level

Fig. 3

FRα CAR T cells secrete Th1 cytokines and up-regulate CD137 in response to FRα (+) tumor cells. a IFN-γ secretion of FRα CAR-transduced T cells after 20 h co-culture with the indicated tumor lines at a 1:1 ratio. Untransduced (UNT) T cells were used as a negative control. b Antigen-specific T cell activation was detected by the induction of CD137 expression. c FRα CAR T cells were stimulated with MDA-231 cells for 5 h in the presence of Golgi stop and analyzed by flow cytometry for intracellular cytokines IFN-γ, TNF-α, and IL-2. UNT T cells served as our negative control, whereas PMA and ionomycin-treated T cells served as positive controls

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