Fig. 4

GSEA in LINC00261 higher/lower expression gastric cancer patients and the correlation between of LINC00261 and its target genes. a GSEA comparing LINC00261 lower expression group (red) against LINC00261 higher expression group (blue) of patients with gastric cancer in the GSE13911 dataset, illustrating distinct pathways and biologic processes between both subgroups. Enrichment plots are shown for a set of activated genes related to cell adhesion in GSE13911 patients’ dataset. The enrichment score (ES, green line) means the degree to which the gene set is overrepresented at the top or bottom of the ranked list of genes. Black bars indicate the position of genes belonging to the gene set in the ranked list of genes included in the analysis. A positive value indicates more correlation with “LINC00261 lower expression” patients and a negative value indicates more correlation with “LINC00261 higher expression” patients. b–d The Pearson correlation analysis of LINC00261 expression with target gene based on GSE13911 patients’ database. LINC00261 is negative related to FN1 (b) and N-cadherin (d), while positive correlated with E-cadherin (c). e, f qPCR analysis of LINC00261 expression levels following the treatment of BGC823 cells with empty vector and pcDNA3.1-LINC00261 (e), and the treatment of MGC803 cells with scrambled siRNA and si-LINC00261 (f). Experiments were performed in triplicate. Bars: SD; *P < 0.05, **P < 0.01