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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Chasing the precursor of functional hematopoietic stem cells at the single cell levels in mouse embryos

Fig. 1

New findings on precursors of HSCs. Hematopoietic stem cells (HSCs) are believed to develop from hemogenic endothelium (EC) cells. The earliest HSCs that are capable of reconstitution potential emerge at embryonic day (E) 10.5 in aorta-gonad-mesonephros (AGM) region, then later migrate to fetal liver, and finally settle in the future adult bone marrow and spleen. The identification of cell surface markers of different stage HSCs facilitate for efficient enrichment of HSCs, thus to elucidate HSC development transcriptional machinery. Recently, CD201 (endothelial protein C receptor, EPCR) was identified as a pre-hematopoietic stem cells (pre-HSCs) surface marker for the first time. By isolation and analyses of pre-HSCs at single cell levels, unique cell-cycle features of pre-HSCs were discovered. Different from past studies that most of adult HSCs reside in G0 phase and none of the S/G2/M phase adult HSCs could successfully reconstitute, transplantation of S/G2/M phase pre-HSCs demonstrates most robust reconstitution capability, G0 phase pre-HSCs show moderate activity, and none of the G1 phase pre-HSCs is able to reconstitute. During the development of HSCs, activation of mammalian targets of rapamycin (mTOR) signaling pathway is fundamental for HSCs emergence

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