Skip to main content

Table 4 Summary of results from sorting and immunomagnetic cell selection MACS

From: Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones

Sample no. Age Total unsorted cells % CD34 unsorted cells % CD123 unsorted cells Total CD34+/CD38−/CD123+ cells from the sorter Total CD34+/CD38−/CD123− cells from the sorter WBC count ×109/L FAB-subtype Cytogenetics % blast % diagnosis Sequencing FLT3/ITD
1 46 10 × 106 96 86 150 1606 5.6 M1 Normal 80 ND
2 60 18 × 106 89 99 76,000 418 4.5 M1 Normal 62 ND
3 69 12 × 106 91 86 12,674 148,396 14.4 M2 Normal 22 78 bp
4 53 27 × 106 73a 93 1839 16 179 M5a Normal 82 33 bp
5 62 30 × 106 10 64 559 164 145 M4 Normal 40 ND
6 65 17 × 106 15 99 300,000 69 6.11 M2 Normal 73 ND
7 72 40 × 106 79 68 640 3602 0.92 M1 Normal 26 ND
  1. Total unsorted cells represents the starting sample size, whereas total CD34+CD38−CD123+ and CD34+CD38−CD123− cells refers to the total number of CD34+/CD38−/CD123+/− cells obtained from a given sample after sorting. Genomic DNA was sequenced for FLT3/ITD for sample nos. 3 and 4. ND indicates not done
  2. aAfter CD34+ selection by MACS, the total yield of CD34+ = 2.4 × 10−5 cells