Table 4 Summary of results from sorting and immunomagnetic cell selection MACS
From: Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones
Sample no. | Age | Total unsorted cells | % CD34 unsorted cells | % CD123 unsorted cells | Total CD34+/CD38−/CD123+ cells from the sorter | Total CD34+/CD38−/CD123− cells from the sorter | WBC count ×109/L | FAB-subtype | Cytogenetics | % blast % diagnosis | Sequencing FLT3/ITD |
|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 46 | 10 × 106 | 96 | 86 | 150 | 1606 | 5.6 | M1 | Normal | 80 | ND |
2 | 60 | 18 × 106 | 89 | 99 | 76,000 | 418 | 4.5 | M1 | Normal | 62 | ND |
3 | 69 | 12 × 106 | 91 | 86 | 12,674 | 148,396 | 14.4 | M2 | Normal | 22 | 78 bp |
4 | 53 | 27 × 106 | 73a | 93 | 1839 | 16 | 179 | M5a | Normal | 82 | 33 bp |
5 | 62 | 30 × 106 | 10 | 64 | 559 | 164 | 145 | M4 | Normal | 40 | ND |
6 | 65 | 17 × 106 | 15 | 99 | 300,000 | 69 | 6.11 | M2 | Normal | 73 | ND |
7 | 72 | 40 × 106 | 79 | 68 | 640 | 3602 | 0.92 | M1 | Normal | 26 | ND |