Fig. 2
Enhancement effect of MACC1 on the Warburg effect in HER2-positive GC cell lines. a, b Glucose uptake (a) and lactate production (b) of the cells was measured 24 h after NCI-N87 and MKN45 MACC1 overexpression or downregulation cells and their control (vector and scramble). NC, NCI-N87, or MKN45 parental cells. Cells seeded in 96-well plates at 5 × 103 cells/well, respectively. Data represent mean ± SD of triplicate experiments,*P < 0.05, # P < 0.01, + P < 0.001. c Western blot analysis of protein extracts from NCI-N87 and MKN45 cells 48 h after transient transfected by MACC1 or vector. GAPDH was used as a loading control. d Western blot analysis of protein extracts from NCI-N87 and MKN45 cells 48 h after transient transfected by siMACC1 or siCtrl. GAPDH was used as a loading control. e Western blot analysis of protein extracts from NCI-N87/TR and MKN45/TR cells 48 h after transient transfected by siMACC1 or siCtrl. GAPDH was used as a loading control