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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: A new mechanism of trastuzumab resistance in gastric cancer: MACC1 promotes the Warburg effect via activation of the PI3K/AKT signaling pathway

Fig. 4

MACC1 promoted the Warburg effect and induced resistance to trastuzumab via PI3K/AKT pathway. a Western blot analysis of protein extracts from NCI-N87 and MKN45 cells 48 and 72 h after treated by Ttzm (40 μg/ml). GAPDH was used as a loading control. b Western blot analysis of protein extracts from NCI-N87 and MKN45 cells 48 h after transient transfected by MACC1 or vector, siMACC1 or siCtrl. GAPDH was used as a loading control. c Western blot analysis of protein extracts from NCI-N87 and MKN45 MACC1 overexpression or vector transfected cells, 24 h after treated with 1 μM MK2206. GAPDH was used as a loading control. d Glucose uptake (red) and lactate production (blue) of the cells was measured 24 h after NCI-N87 and MKN45 MACC1 overexpression plus or not plus treated with 1 μM MK2206. Cells were seeded in 96-well plates at 5 × 103 cells/well, respectively. NC, NCI-N87, or MKN45 parental cells. Data represent mean ± SD of triplicate experiments, *P < 0.05, # P < 0.01, + P < 0.001.e MTT assays were performed 72 and 96 h after Ttzm (10 μg/ml) treatment of the indicated cells. Data represent mean ± SD of triplicate experiments relative to untreated cells. Inhibition of viability was compared between MACC1 and MACC1 + MK2206 group by multiple t test. *P < 0.05, # P < 0.01, + P < 0.001. f Western blot analysis of protein extracts from NCI-N87 and MKN45 cells 48 h after co-transfected with siMACC1 and Myr-AKT or siCtrl and Myr-AKT. GAPDH was used as a loading control. g Glucose uptake (red) and lactate production (blue) of the cells were measured 24 h after NCI-N87 and MKN45 siMACC1/siCtrl plus or not plus Myr-AKT co-transfected. Cells seeded in 96-well plates at 5 × 103 cells/well, respectively. NC, NCI-N87, or MKN45 parental cells. Data represent mean ± SD of triplicate experiments, *P < 0.05, # P < 0.01, + P < 0.001. h MTT assays were performed 72 and 96 h after Ttzm (10 μg/ml) treatment of the indicated cells. Data represent mean ± SD of triplicate experiments relative to untreated cells. Inhibition of viability was compared between siMACC1 and siMACC1 + Myr-Akt group by Student’s t test.*P < 0.05, # P < 0.01, + P < 0.001

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