Fig. 2

Comparison of NRF2-regulated gene expression in NSCLC cells exposed to platinum-based drugs. a, b The levels of HO1, NQO1, and GCLM mRNA expression in H292 and SKMES-1 cells exposed to 30 μM nedaplatin or cisplatin for 24 h. c, d The expression levels of HO1, NQO1, and GCLM mRNA in A549 and SKMES-1 cells exposed to 30 μM nedaplatin or cisplatin for 24 h. Comparison was conducted between 24-h points and control group by using Student’s tests. The results are presented as the mean ± SEM. *p < 0.01, **p < 0.05. e, f The levels of HO1, NQO1, and GCLM protein expression in H292 and SKMES-1 cells exposed to 30 μM nedaplatin or cisplatin for 3 or 24 h. g, h The levels of HO1, NQO1, and GCLM protein expression in A549 and H460 cells exposed to 30 μM nedaplatin or cisplatin for 3 or 24 h. i Immunofluorescence staining of HO1 in H292 (upper panel) and SKMES-1 (lower panel) cells exposed to 30 μM nedaplatin or cisplatin over a 24-h treatment period. j Immunofluorescence staining of HO1 in H460 (upper panel) and A549 (lower panel) exposed to 30 μM nedaplatin or cisplatin over a 24-h treatment period