Table 1 Characteristics of patients in study group and historical group (n = 81)

Patients’ number

47

34

Age (years, range)

28 (4-58)

25 (7-57)

0.696

Gender

0.294

 Male (%)

29 (62)

17 (50)

 Female (%)

18 (38)

17 (50)

Diagnosis (%)

0.300

 Acute myeloid leukemia

25 (53)

22 (65)

 Acute lymphoid leukemia

22 (47)

12 (35)

Remission state pre-HSCT (%)

0.154

 CR1

39 (83)

25 (73)

 CR2

5 (11)

9 (27)

 CR3

2 (4)

0 (0)

 NR

1 (2)

0 (0)

Cytogenetic subgroups^a (%)

0.285

 Intermediate

30 (64)

18 (53)

 Poor

10 (21)

6 (18)

 Not available

7 (15)

10 (29)

Numbers of induction chemotherapies (%)

0.214

 ≤2

39 (85)

25 (74)

 >2

7 (15)

9 (26)

Donor types (%)

0.037

 HLA-identical related

11 (23)

15 (44)

 Haploidentical related

31 (66)

19 (56)

 Unrelated

5 (11)

0 (0)

HLA-mismatch (%)

0.230

 0 locus mismatch

4 (11)

0 (0)

 1 locus mismatch

3 (8)

4 (21)

 2 locus mismatch

10 (28)

7 (37)

 3 locus mismatch

19 (53)

8 (38)

Donor-patient sex match (%)

0.377

 Female-female

3 (6)

6 (18)

 Female-male

13 (28)

7 (21)

 Male-male

18 (38)

14 (41)

 Male-female

13 (28)

7 (21)

ABO match (%)

0.994

 Match

31 (66)

22 (65)

 Major mismatch

6 (13)

5 (15)

 Minor mismatch

6 (13)

4 (12)

 Major and minor mismatch

4 (9)

3 (9)

Conditioning regimen (%)

0.635

 TBI-based

3 (6)

1 (3)

 Bu-based

44 (94)

33 (97)

Acute GvHD of grades 2–4 pre-DLI

14 (30)

9 (27)

0.744

Acute GvHD of grades 3–4 pre-DLI (%)

4 (9)

0 (0)

0.135

Chronic GvHD pre-DLI (%)

10 (21)

12 (35)

0.162

Moderate or severe chronic GvHD pre-DLI (%)

4 (9)

6 (18)

0.307

Interval from HSCT to relapse (day) (range)

204 (39-2180)

241 (40-2405)

0.737

BM blasts at the time of relapse (%) (range)

29 (7-93)

28 (9-92)

0.513

Chimerism at the time of relapse (%)

0.294

 Full-donor chimerism

34 (72)

28 (82)

 Partial-donor chimerism

13 (28)

6 (18)

Times of DLI after induction chemotherapy plus DLI

NA

 1

21 (45)

0 (0)

 2

18 (38)

0 (0)

 3

7 (15)

0 (0)

 4

1 (2)

0 (0)

MNCs in DLI (×10⁸/kg, range)

1.00 (1.00-1.50)

1.00 (0.70-1.70)

0.957

CD3⁺ cells in DLI (×10⁸/kg, range)

0.34 (0.15-0.64),

0.37 (0.15-0.74),

0.653

CD4⁺ cells in DLI (×10⁸/kg, range)

0.20 (0.10-0.42)

0.23 (0.09-0.31)

0.732

CD8⁺ cells in DLI (×10⁸/kg, range)

0.12 (0.06-0.28)

0.14 (0.05-0.31)

0.657

CD14⁺ cells in DLI (×10⁸/kg, range)

0.26 (0.04-0.48)

0.29 (0.09-0.61)

0.432

CD34⁺ cells in DLI (×10⁶/kg, range)

0.48 (0.14-1.44)

0.54 (0.12-1.58)

0.354