Fig. 4

Statistical analysis of surface marker expression on developing cells during iPSC differentiation to hematopoietic cells in 3D induction systems. Cells were harvested from 3D induction cultures on the indicated days and processed for flow cytometry assay to determine the committed cell properties. Statistic analyses showed that the expression or coexpression patterns of CD34, CD45, CD117, CD31, CD43, CD38, CD144/VE-cadherin, CD133, CD90, and CD309/KDR presented on a subpopulation of different phase population, indicating the commitment of the hemangioblasts, hemogenic endothelium, hematopoietic stem cells, and hematopoietic progenitors in the cultures. Data were represented with mean ± SEM; n = 3