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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: A synthetic three-dimensional niche system facilitates generation of functional hematopoietic cells from human-induced pluripotent stem cells

Fig. 6

Production of mature hematopoietic cells in the following 3D culture systems. (Aa–Dc) The morphology was captured during the culture for 21–45 days. Some colonies were formed similar to CFUs in Methocult 4434 (A, B, and C). The relevant bright, immunofluorescent and overlapped field visions were shown in a, b, and c, respectively. Some cells were similar to the subtypes of lymphoid cells in morphology as shown in Da–Dc. E–H Kinetic expression of CD3+, CD19+, CD45+, and CD34+ cells to analyze the multi-lineage potential of CD34+ hematopoietic stem cells derived from iPSCs in vitro with flow cytometry assay. I Statistics analysis of CD3+, CD19+, CD45+, and CD34+ cells in 3D later induction system. Error bars represented mean ± SEM, n = 3

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