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Fig. 7 | Journal of Hematology & Oncology

Fig. 7

From: A synthetic three-dimensional niche system facilitates generation of functional hematopoietic cells from human-induced pluripotent stem cells

Fig. 7

Transplantable potential analysis of iPSC-derived hematopoietic cells in conditioned NOD/SCID mice. a Schematic representation of xenogenic transplantation mouse model and iPSC-derived hematopoietic cell transplantation. b–r FACS analysis revealed the presence of human blood cells, myeloid cells, B cells, T cells, and CD235a + erythroid cells in the transplanted mice. The percentage of each population was indicated for a representative mouse. b–e Human CD45+CD3+ cells, CD45+CD15+ cells, and CD45+CD19+ cells were detected in the peripheral blood of the transplanted mice. f–i Human CD45+CD3+ cells, CD45+CD15+ cells, and CD45+CD19+ cells were present in the spleens of the animals. j–n Human CD45+CD3+ cells, CD45+CD15+ cells, and CD45+CD19+ and CD45+CD34+ cells were present in the bone marrow. o, p The CD235a+ population was shown as the percentage compared with the total blood cell number. q, r CD3+ cells, gated on Tra-1-85+ cells, were present in thymuses of the transplanted mice, indicating that human T cells had entered the thymuses. s Different blood populations were analyzed in the transplanted mice. The value was the mean ± SEM of six independent experiments. t The results of RT-PCR showed that human-specific mitochondria production was detected in the cells sorted from the transplanted animals with human CD45 antibody staining. Lanes 1–2: amplification of human CD45+ cells sorted from the transplanted mice using human-specific primers, indicating that human hematopoietic cells presented in the transplanted animals. Lane 3–4: demonstration of the mouse DNA control using mouse-specific primers. Lane 5: DNA ladder markers. Lane 6: negative control with water. CTX cyclophosphamide, i.p. intraperitoneal, PB peripheral blood, BM bone marrow, SP spleen

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