Table 1 Performance of RQPCR by consensus germline or entirely patient-specific primers/probes
Cases | Consensus germline primers/probes | Patient-specific primers/probes | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diagnostic marrow DNA as standards | Diagnostic marrow DNA as standards | CDR3-cloned plasmid as standards | ||||||||||
Sensitivity | QR | R 2 | Slope | Sensitivity | QR | R 2 | Slope | Sensitivity | QR | R 2 | Slope | |
Dx53 | N/A | 10−2 | N/A | N/A | 10−5 | 10−4 | 0.996 | −3.30 | 10−5 | 10−4 | 0.992 | −3.44 |
M3 | ND | ND | ND | ND | 5 × 10−4 | 5 × 10−4 | 0.989 | −3.48 | 10−4 | 10−4 | 0.997 | −3.54 |
M8 | 10−4 | 5 × 10−4 | 0.985 | −3.38 | 10−4 | 10−3 | 0.999 | −3.46 | 10−4 | 5 × 10−4 | 0.999 | −3.61 |
M4 | ND | ND | ND | ND | 10−4 | 10−3 | 0.984 | −3.60 | 10−5 | 10−3 | 0.999 | −3.54 |
Dx31 | ND | ND | ND | ND | 10−4 | 5 × 10−4 | 0.998 | −3.45 | 10−4 | 10−4 | 0.998 | −3.47 |
M5 | 10−4 | 10−4 | 0.995 | −3.41 | 5 × 10−4 | 5 × 10−4 | 0.995 | −3.67 | 10−5 | 10−4 | 0.998 | −3.51 |
M10 | N/A | 5 × 10−4 | N/A | N/A | N/A | 5 × 10−4 | N/A | N/A | 10−4 | 10−4 | 0.994 | −3.51 |
Dx49 | 5 × 10−4 | 10−3 | 0.982 | −3.37 | 10−4 | 10−3 | 0.986 | −3.26 | 10−4 | 5 × 10−4 | 0.999 | −3.76 |
M1 | ND | ND | ND | ND | 5 × 10−4 | 10−3 | 0.986 | −3.48 | 10−4 | 5 × 10−4 | 0.998 | −3.52 |
M6 | ND | ND | ND | ND | N/A | 5 × 10−4 | N/A | N/A | 10−5 | 5 × 10−4 | 0.997 | −3.72 |
M2 | N/A | N/A | N/A | N/A | ND | ND | ND | ND | ND | ND | ND | ND |
M7 | N/A | 10−2 | N/A | N/A | N/A | 10−2 | N/A | N/A | 10−4 | 10−3 | 0.999 | −3.56 |
M9 | N/A | 10−2 | N/A | N/A | N/A | 10−2 | N/A | N/A | 10−4 | 10−4 | 0.997 | −3.55 |