DNMT3A R882 mutation enhances cell migration in vitro.
a Transwell assays of acute leukemia cell lines OCI-AML3, THP-1, Kasumi-1, U937, and NB4. Each strain is seeded in a number of 1 × 104 cells. Data are expressed as mean ± SD; n = 6 per group. b Transwell assays of OCI-AML3 with or without DNMT3A mRNA knockdown. About 1 × 104 of cells are purified for inoculation. Expression levels of DNMT3A proteins are shown in the left panel. Data are presented as mean ± SD for each group (n = 7). c Transwell assays of U937 cells that are stably transfected by lentivirus with wild-type (WT) DNMT3A or DNMT3A R882C. Overexpressed WT and mutant DNMT3A are indicated by Flag antibody in the upper panel. Approximately 1 × 104 of purified cells is used for plating in each well. Data are expressed as mean ± SD (n = 6 per group). d Scratch-wound assays of MDA-MB-231 cells stably expressing the vector of WT DNMT3A or DNMT3A R882C tagged by Flag. Upper panel shows the exogenous DNMT3A expression by anti-Flag. Representative images in lower panel are shown at 0 and 12 h after scratching