Fig. 2
From: DNMT3A mutation leads to leukemic extramedullary infiltration mediated by TWIST1
Extramedullary infiltration of OCI-AML3 cells in mice. a Representative NOD/SCID mouse transplanted with OCI-AML3 cell line presents paralysis syndrome at 1Â month post xenografting. Red arrows indicate the paralyzed hind limbs. b PET and CT images show leukemia cells infiltration in murine spinal cords. Radioactive substrates distributed in bladders are shown as the internal controls. c Bioluminescent imaging of NOD/SCID mice at 1Â month post xenografting. OCI-AML3 cells with luciferase are distributed mainly in the brain, spinal cord, and BM. d Representative FACS plots of digested cells from control and OCI-AML3-, U937-, and THP-1-xenografted murine brains, where human CD44-positive (hCD44+) human sources cells are gated to indicate