Fig. 2

Effect of BKM120 and BEZ235 on T cell proliferation and apoptosis induction. PBMCs (a) or isolated T cells (b) were stimulated and treated with different concentrations of BKM120 or BEZ235. a Percentage of T cells that had undergone one or more cell divisions (CD3⁺PKH^low) after 5 days of culture. b Percentage of T cells in synthesis and G2/mitosis (S-G2/M) phases after 4 days of culture. #p < 0.05 with respect to untreated control (0 μM). Percentage of annexin V⁺ cells among unstimulated (c) or stimulated (d) isolated T cells. Data pooled from six independent experiments. Outliers are represented by circles (values > 1.5 × IQR) and stars (values > 2 × 1.5 × IQR). IQR, interquartile range. e Western blot analysis of cleaved caspase 3 in T cell-enriched PBMCs. Results representative from three independent experiments