Skip to main content
Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: T cells in multiple myeloma display features of exhaustion and senescence at the tumor site

Fig. 2

CD8+ T cells of myeloma patients show functional defects in proliferation and cytotoxicity. a Proliferation was assessed by 3H-thymidine incorporation after stimulating the cells for 72 h with anti-CD3/anti-CD28 antibodies. The proliferative index (3H-thymidine incorporation of stimulated T cells divided by 3H incorporation of unstimulated cells) is shown for BM CD8+ T cells from myeloma patients (n = 12) and from healthy controls (n = 6). b T-bet and c IFNγ expression was assessed by intracytoplasmic staining of total CD8+ BMMCs before (0 h) and after stimulation (24 h) of the cells (n = 7; median with IQR is shown). d T-bet and IFNγ expression on different T cell subtypes (CD8+ CD45RA+ or CD45RA−) was assessed by intracytoplasmic staining before (0 h) and after (24 h) stimulation with CD3/CD28 antibodies (n = 7; mean ± SEM is shown). e Surface levels of CD107a were detected by flow cytometry before (0 h) and after stimulation (24 h) (n = 3; median with IQR is shown). Significance levels: *p < 0.05; **p < 0.01; n.s. not significant

Back to article page