Fig. 3

H19 and HULC regulated genes via ceRNA behaviors. a Schematic representation of the interaction between miRNAs and the 3'-UTR of IL-6 or CXCR4. b Let-7a/let-7b and miR-372/miR-373 reduced the abundance of IL-6 and CXCR4 proteins, respectively in CCA cells. The right histogram analyzed the relative fold charges of protein levels. Triplicate experiments were analyzed by mean ± SD, p value <0.01, **, <0.05, *. MiRNA down-regulation by transfecting the miRNA inhibitors into RBE cells under oxidative stress, and the expression levels of IL-6 (c) and CXCR4 (d) increased significantly. Enforced expressed of H19 and HULC rescued luciferase activity for vectors with the 3'-UTR of IL-6 or CXCR4, which were suppressed by let-7a/let-7b and miR-372/miR-373, respectively, in RBE cells (e), and in HEK-293 T cells (f)