Fig. 4

The mechanism of H19 and HULC that regulated genes. a AGO2 rip-qPCR of miRNAs and lncRNAs in RBE cells. The enrichment of miRNAs binding with AGO2 were used as the positive controls and U6 as the negative. b AGO2 rip-qPCR of miRNAs and lncRNAs in QBC939 cells. Triplicate experiments were analyzed by mean ± SD, p value <0.001, ***. c Relative enrichment of H19 (green) and HULC (blue) were analyzed in AGO2 rip-qPCR with let-7a, let-7b inhibitor or miR-372, miR-373 that compared to inhibitor NC. Enforced expression of different concentration of H19 (d) and HULC (e) plasmid increased IL-6 and CXCR4 protein levels, respectively, in RBE cells. The expression of IL-6 (f) and CXCR4 (g) had decreased significantly when we knocked down H19 and HULC, respectively, under the oxidative stress condition. The right histogram of gray density to quantitatively analyze the relative fold charges of protein levels. Triplicate experiments were analyzed by mean ± SD, p value < 0.001, ***, <0.01, **, <0.05, *