Fig. 4
The Wnt/β-catenin pathway contributes to the high MYC expression in RR cells. a The relative mRNA levels of WNT2B, CTNNB1, LEF1, MYC, and BCL9 in RU and RR cells derived from SupM2 and Karpas 299, assessed by qRT-PCR. b The protein levels of active β-catenin, β-catenin, pGSK3βS9, GSK3β, LEF1, and Sox2 in RU and RR cells. c The SRR2 luciferase activity in RR cells with scr siRNA or β-catenin siRNA transfection at 48 h. The western blots below showed the knockdown efficiency of β-catenin and MYC. d The SRR2 luciferase activity in RR cells treated with DMSO or 50 μM quercetin, a pharmacological β-catenin inhibitor for 24 h. The western blots below showed the knockdown efficiency of β-catenin and MYC. e The diagram showed the design of transwell co-culture experiment. f The SRR2 luciferase activity in RU cells in the control group and the experimental group (Exp-group). g The western blots showed the protein levels of β-catenin, LEF1, and MYC in RU cells derived from SupM2 in the control group and experimental group